English
聯系我們
網站地圖
郵箱
舊版回顧



城市環境所利用單細胞拉曼光譜研究土壤解磷微生物取得進展

文章來源:城市環境研究所   發布時間:2019-05-10  【字號:     】  

  磷是一種至關重要但不可再生的資源。磷肥是作物生長必須三大營養元素(氮、磷、鉀)之一,但磷礦是有限資源,據估計,以目前的磷礦儲備,大概還能繼續開采100年,因此,磷又被稱為“正在消失的元素”。隨著長期大量的磷肥施用,土壤中積累了大量固定態磷,大大降低了磷素的生物有效性,加劇了磷資源短缺的問題,對土壤固定態磷的活化成為未來發展可持續農業和磷肥利用的重要戰略。

  微生物是土壤元素循環的發動機,解磷微生物可以通過釋放有機酸溶解土壤中的鈣磷,或釋放活性磷酸酶活化土壤中的有機磷。利用解磷微生物釋放土壤中被固定的磷,提升磷的生物有效性,對磷素的可持續利用和緩解磷礦枯竭危機具有至關重要的作用。然而,環境微生物的大量不可純培養性,對開發利用微生物資源帶來了很大的挑戰。從單細胞水平上研究環境微生物可克服純培養或富集培養的限制,實現在環境介質下的原位研究,拉曼光譜可在單細胞水平上對微生物進行無損檢測。

  中國科學院城市環境研究所朱永官研究團隊發展了利用單細胞拉曼結合重水標記篩選高活性土壤解磷微生物的新方法。該方法可以無損檢測土壤環境中不可培養的微生物,從而挖掘出具有強解磷功能的微生物。方法原理是:在僅固定態無機磷(如難溶磷酸三鈣)或有機磷(如卵磷脂)存在的環境中,具有解磷功能的微生物由于仍具活性,甚至生長繁殖能力,因此能同化重水中的氘,產生特征的C-D拉曼峰。解磷微生物與非解磷微生物在C-D拉曼峰存在巨大的強度差異,解磷能力越強的微生物具有越強的C-D拉曼峰,利用這一特性可以靈敏快速地篩選出土壤中的解磷微生物。重水標記由于不改變環境營養底物庫,可更真實反映土壤環境中的解磷微生物。上述工作促進了對未知環境菌群的深入認識,尤其是磷循環功能菌及其活性的深入解析。研究成果以D2O Isotope Labeling Approach to Probing Phosphate Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy 為題發表在國際期Analytical Chemistry,城市環境所博士生李弘哲為第一作者,研究員崔麗為通訊作者。該研究得到科技部國家重點研發計劃和國家自然科學基金等的資助。

  文章鏈接 

 

土壤解磷菌拉曼鑒定流程

 

無磷土壤中培養24小時的土壤細菌的單細胞拉曼光譜




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

百姓彩票www.bxcp3.com www.930027.com | www.18uq.com | www.716881.com | www.914492.com | www.53653.cc | www.837664.com | www.823444.com | 500彩票www.50052v.com | www.0348.vip | www.052931.com | www.214881.com | www.113991.com | www.871605.com | www.7711.me | www.160558.com | www.720670.com | 672250.com | www.085303.com | www.88383.com | www.97188.com | www.373206.com | www.998139.com | www.29xi.com | www.37fg.com | www.114102.com | 865878.com | www.262536.com | www.220327.com | 023471.com | www.c97.club | www.182766.com | www.z61.org | www.3065.com | www.917818.com | www.732396.com | www.82td.com | www.132126.com | 公益福彩www.gyfc1.com | www.046.online | www.234908.com | www.257384.com | 421664.com | www.652666.cc | www.987117.com | www.531608.com | www.762300.com | www.090071.com | www.251952.com | www.365719.cc | www.927468.com | www.841447.com | www.75if.com | www.012126.com | www.7800.net | www.59211.com | www.753025.com | www.857685.com | www.130567.cc | 320335.com | www.54ed.com | www.024791.com | www.063555.com | www.042969.com | www.473002.com | www.371790.com | www.372596.com | www.993074.com | 134798.com | 073335.com | www.996040.com | 90486.com | 365350.cc | www.280881.com | www.977184.com | 546908.com | 634759.com | 560886.com | www.85rk.com | www.181728.com | www.515979.com | www.518436.com | www.569708.com | www.4867.cn | www.706538.com | www.857563.com | 029754.com | www.84jm.com | www.3103.cm | www.150061.com | www.796783.com | www.603.org | www.201145.com | www.288219.com | www.310122.com | 188730.cc | www.v65.org | www.161009.com | www.3208.cm | www.965543.com | 673665.cc | www.05581.com | www.178442.com | 788564.com | www.05242.com | www.xq75.com | www.12801.com | www.745723.com | 920999.com | www.424120.com | www.177177.cc | www.877842.com | www.289296.com | 369794.com | www.85az.com | www.8kv.com | www.11445.com | www.130480.com | 026161.com | 亚投彩票www.299058.com | www.17720.com | 458837.com | 852693.com | 818合彩www.www.5666hc.com | www.5937.biz | www.025996.com | www.98745.cc | www.141588.com | www.323477.com | 博乐彩票www.35918c.com | www.592850.com | 821644.com | 981039.com | www.99081.cc | www.562815.com | www.551268.com | www.510496.com | www.989927.com | www.858255.com | www.235880.com | www.450396.com | www.47ru.com | www.166889.com | www.617519.com | 3504.cc | 340955.com | www.am62.cc | www.363984.com | 815080.com | www.478152.com | www.979424.com | 129924.com | www.0079.cm | www.211446.com | www.713202.com | www.820852.com | www.982933.com | 809014.com | www.040896.com | www.483245.com | www.666163.com | 890560.com | www.352851.com | www.975.date | www.19335.cc | www.454518.com | www.874033.com | www.hx95.com | www.478895.com | www.489554.com | www.666468.com | www.7036.shop | www.88678.com | 981930.com | www.273594.com | www.601954.com | www.388466.com | www.899.date | www.051759.com | www.sk59.com | www.830832.com | www.112664.com | www.213349.com | www.339086.com | www.833181.com | www.089079.com | www.481527.com | www.185.red | www.36674.com | www.157687.com | www.008049.com | www.695597.com | 868284.com | www.yb2.cc | www.59450.com | www.352337.com | www.843313.com | www.jo23.com | www.53722.com | www.027843.com | 290540.com | 866209.com | www.89206.com | www.7411.wang | www.59255.com | 60889.com | www.279779.com | www.44068.cc | www.023996.com | www.137787.com | www.095111.com | www.745877.com | 111455.com | www.267172.com | 955944.cc | www.50066.com | 928804.com | www.q31.net | www.897129.com | 117609.com | zw31.com | www.492675.com | www.481300.com | www.431274.com | www.822323.com | www.ni15.com | www.026235.com | 299231.com | www.l52.top | www.06366.com | www.143570.com | www.394545.com | www.818041.com | www.586310.com | 237645.com | 大赢家彩票网www.565121.com | www.276909.com | www.428244.com | www.b72.com | www.836254.com | www.6950.vip | www.ck03.com | www.616469.com | www.905549.com | 751843.com | www.111311.com | 245249.com | www.rl69.com | www.44ks.com | www.949427.com | www.410754.com | www.35zo.com | www.28iv.com | www.027.red | 659443.com | www.426223.com | www.580708.com | www.8537.xyz | www.013681.com | www.432772.com | www.691965.com | www.31iq.com | www.10219.com | www.369554.com | 240516.com | www.lt39.com | www.9475.cn | www.013547.com | www.199111.com | www.989.org | www.500031.cc | www.337252.com | 651506.com | 380035.com | 590261.com | www.89095.com | www.950305.com | 604447.com | www.3227.shop | www.647200.com | www.22308.com | www.3878.biz | www.246344.com | www.532980.com | www.ib05.com | www.567095.com | www.548578.com | www.952256.com | www.927153.com | www.42803.com | www.515759.com | www.12615.com | www.061477.com | www.237672.com | www.337385.com | www.720006.com | 202312.com | www.112049.com | www.735073.com | www.57833.com | www.312662.com | www.294363.cc | www.636157.com | www.14115.com | 736126.com | www.089024.com | www.152.pw | www.245885.com | www.854818.com | www.996618.com | www.339869.com | www.r93.in | www.822835.com | www.kh14.com | www.16em.com | www.00aw.com | www.04qt.com | www.656261.com | www.280542.com | 868874.com | 503308.com | www.34sc.com | www.181991.com | 039803.com | 大赢家彩票平台www.591023.com | www.xg87.com | www.69wi.com | www.306786.com | www.3332.top | 43hz.com | www.224033.com | www.22zx.com | www.899146.com | www.642273.com | 039119.com | www.136702.com | 33979.com | 857023.com | www.720390.com | 739270.com | www.451177.com | www.331473.com | www.860765.com | 718312.com | 422020.com | www.421500.com | www.37gi.com | www.82173.com | www.733731.com | www.65355.cc | www.ls66.com | www.10338.com | 594775.com | 706448.com | www.ew24.com | www.041001.com | 华彩www.71399t.com | www.0288.net | www.223185.com | www.213579.com | 412377.com | www.sf22.cc | www.4817.org | 791439.com | www.969617.com | www.85su.com | www.8344.com | www.759513.com | www.2446.in | www.447326.com | www.088.link | www.003123.com | www.32510.com | www.725615.com | 443886.com | www.024421.com | www.xf01.com | www.773964.com | www.0515.tv | www.580169.com | www.db05.com | www.eb38.com | www.9911.loan | www.301434.com | www.9vf.com | www.od42.com | www.822664.com | www.088095.com | www.964650.com | 8620.biz | www.60074.com | www.9944.cn | www.26869.cc | www.950481.com | 526039.com | 586984.com | www.121239.com | www.834408.com | www.081097.com | www.400947.com | www.08364.com | www.435178.com | 68彩票www.68682i.com | www.fl4.cc | www.61nn.com | www.40jq.com | www.528071.com | www.or93.com | www.123866.com | www.652139.com | www.613629.com | www.636231.com | 994875.com | www.169944.com | www.902496.com | www.728903.com | www.720900.com | www.dn45.com | 超级彩票www.976506.com | www.em02.com | 562865.com | 964994.com | www.9338.loan | www.676509.com | www.857685.com | 369384.com | 755548.com | 199584.com | 七星彩票www.015689.com | www.24cx.com | www.558124.com | www.526865.com | www.651192.com | www.12113.com | www.044389.com | 盛彩网www.41518r.com | www.807848.com | 588900.com | www.280694.com | www.071556.com | www.788778.com | www.38ib.com | www.475989.com | www.999190.com | www.959704.com | www.18xs.com | www.ae03.com | 华彩网www.hcw332.com | 大玩家彩票www.84499j.com | www.b31.vip | www.056516.com | www.433658.com | www.355.date | www.25217.cc | www.094618.com | www.147002.com | www.09vt.com | www.023176.com | 089024.com | 082796.com | www.110398.com | www.656677.com | www.539968.com | www.3710.vip | www.4786.cm | www.9001.website | www.769305.com | www.950297.com | www.2989.com | www.007231.com | www.339723.com | www.in75.com | www.895784.com | www.70158.cc | www.3946.win | www.907182.com | www.97358.cc | www.113734.com | www.rv78.com | www.764702.com | www.318614.com | www.hp46.com | www.938718.com | www.433330.com | www.308019.com | www.981104.com | www.56161.com | www.809494.com | www.176570.com | www.36178.cc | www.8202.top | www.608961.com | www.360574.com | www.yu01.com | www.4te.com | www.m69.me | www.67jn.com | www.827002.com | www.cp6632.com | www.340595.com | www.885110.com | 847723.com | www.4374.cn | www.r35.cn | www.720005.com | www.31wc.com | www.930978.com | www.zt73.com | www.722027.com | 022271.com--135888.com | www.911.bid | www.90903.com | www.378498.com | www.956571.com | www.r44.cc | www.cp6550.com | www.118151.com | www.888216.com | www.213106.com | www.387108.com | www.344327.com | www.285233.com | www.uj68.com | www.mo55.com | www.577282.com | www.25mf.com | www.ba25.com | www.281965.com | www.400289.com | www.zu53.com | 980745.com | www.805789.com | www.724933.com | www.984270.com | www.214244.com | www.593869.com | www.978458.com | www.512122.com | www.0732.live | www.305344.com | www.262693.com | www.234247.com | www.771244.com | www.106033.com | www.109710.com | www.447022.com | www.431998.com | www.837629.com | www.980012.com | www.946665.com | www.910661.com | www.946398.com | www.853839.com | www.211504.com | www.771272.com | www.6418.cc | www.566896.com | www.c58.club | www.998712.com | www.979006.com | www.76qn.com | www.h98.club | www.388712.com | www.jx51.com | www.fk82.com | www.54278.com | www.20516.cc | www.447559.com | www.663425.com | www.738883.com | www.791353.com | www.213772.com | www.23650.com | www.581557.com | www.503726.com | 886484.com | www.cp3110.com | www.10414.com | www.73295.com | 025562.com | www.17646.com | www.236307.com | www.38408.com | www.8910.space | www.067658.com | www.871221.com | www.5056.xyz | www.699275.com | www.19853.cc | www.183917.com | 博乐彩票www.588229.com | www.789058.com | www.mw30.com | www.26jt.com | www.86581.cc | www.764433.com | www.634523.com | 500万彩票www.96386s.com | 059839.com | www.2467.me | www.9169.vip | 120621.com | 158859.com | 276270.com | www.100527.com | www.645107.com | www.479813.com | 029748.com | www.11nn.com | 579032.com | www.4460.in | www.86ou.com | www.533189.com | 984171.com | 059980.com | www.892186.com | www.859206.com | www.8685.cc | www.493377.com | www.832906.com | www.16524.cc | www.037660.com | www.992513.com | www.982431.com | www.813627.com | www.783608.com | www.31oj.com | www.8566.in | 110687.com | 672595.com | 015331.com | www.887372.com | 120871.com | www.731210.com | 672559.com | 128508.com | cai4789.com | www.32kp.com | www.84yz.com | www.579624.com | www.440349.com | 042866.com | 凤凰彩票www.77802s.com | www.48fu.com | www.88hc.com | www.272591.com | www.373651.com | 037316.com | www.106984.com | www.739627.com | www.0434.cc | www.022166.com | 1693.com | www.170009.com | www.187740.com | www.096233.com | www.092424.com | www.1235.net | www.53bl.com | www.590169.com | www.9659.loan | www.385709.com | www.858400.com | www.cp470.com | 350803.com | 009459.com | www.974458.com | www.736006.com | 791746.com | www.190780.com | www.237335.com | www.257806.com | www.969391.com | www.x12.space | www.428280.com | www.ev42.com | www.280860.com | www.967024.com | www.893052.com | 498654.com | www.069225.com | 974836.com | www.811648.com | www.02428.com | www.44310.com | www.53372.com | www.296118.com | 560519.com | 576479.com | www.32mh.com | www.g25.com | 幸运彩票www.5095g.com | www.5916.com | www.964117.com | www.046278.com | www.pt26.com | www.881407.com | www.124028.com | www.p53.com | www.25937.cc | www.862915.com | www.24zi.com | www.tp08.com | www.ym1.cc | www.046847.com | www.289728.com | www.dy08.cc | www.4242.cc | www.339164.com | www.601774.com | www.69075.com | 599448.com | www.768683.com | 348360.com | www.830910.com | www.031.win | www.653516.com | www.46659.com | www.13135.com | www.lq35.com | www.012528.com | 263535.com | www.cp7528.com | www.318470.com | www.319391.com | 637904.com | www.326796.com | www.121568.com | www.572756.com | www.991882.com | www.955431.com | 638794.com | 053964.com | www.56128.cc | www.637448.com | www.329956.com | www.274526.com | www.9102.vip | www.644343.com | 223864.com | 659403.com | 902865.com | www.956087.com | www.707331.com | www.555179.com | www.650172.com | cai2227.com | 129382.com | www.383137.com | www.627356.com | www.847789.cc | www.626590.com | 026932.com | www.654120.com | www.hf25.com | www.156265.com | www.770458.com | www.1395.vip | www.253210.com | www.977424.com | 738598.com | www.649277.com | www.980399.com | www.70ht.com | www.nc57.com | www.xt.cc | www.005085.com | www.030901.com | www.019276.com | www.158270.com | www.609944.com | 611876.com | www.om50.com | www.iz27.com | www.0161.xyz | www.8653.biz | www.85dn.com | www.007564.com | www.98000.com | www.751555.com | www.bu22.com | www.nk32.com | www.8236.me | www.71337.cc | www.599306.com | 256772.com | 394321.com | www.568800.com | www.qw1.cc | www.q32.cc | www.ws29.com | www.79298.com | www.038082.com | www.89628.cc | www.231946.com | www.042920.com | 875235.com | www.gd90.com | www.122802.com | www.75174.com | www.618333.cc | 多乐彩票www.244646.com | www.tr2.com | 669861.com | www.rg47.com | 401965.com | www.169138.com | www.565931.com | www.211488.com | www.424126.com | www.570435.com | www.kv61.com | www.24ej.com | www.67233.com | www.624663.com | www.046589.com | www.318512.com | 943259.com | 880956.com | www.096771.com | 087586.com | www.1si.com | www.089842.com | www.322831.com | www.130166.com | www.600866.com | www.997548.com | www.593779.com | www.66464.cc | www.12zc.com | www.654028.com | 811965.com | www.14145.com | www.l32.net | www.cp4004.com | www.69445.com | www.110124.com | www.770907.com | 979093.com | www.616184.com | www.811913.com | www.566225.com | www.353988.com | www.246278.com | www.85954.com | 418503.cc | www.4034.com | www.sr20.com | www.103365.com | www.17877.cc | www.c19.vip | www.5984.cn | www.289011.com | www.097317.com | www.136261.com | www.623683.com | 43745.com | www.566255.com | www.268092.com | di05.com | www.959434.com | www.782715.com | www.276569.com | 233045.com | www.878759.com | 网易彩票www.082wy.com | www.504585.com | www.oz80.com | www.589308.com | 634183.com | www.302953.com | www.44716.com | www.513960.com | www.29dq.com | www.056198.com | www.2078.cc | www.46hx.com | www.222436.com | www.877991.com | www.34434.com | www.012433.com | www.025770.com | www.839036.com | www.ju40.com | www.23rc.com | www.089784.com | www.24499.com | www.93qb.com | 13677.com | 675199.com | 彩票500万www.708609.com | www.97076.com | www.144856.com | www.g52.club | www.93hv.com | www.7ix.cc | www.t01.org | www.76ju.com | www.228770.com | www.013547.com | www.033798.com | www.7310.me | www.471238.com | www.331052.com | www.207607.com | www.184115.com | 994957.com | www.99037.com | www.925811.com | www.60177.com | www.276481.com | www.60057.cc | www.507788.com | 952702.com | 960756.com | 539310.com | www.276178.com | 365381.cc | www.00543.com | www.16879.com | www.447312.com | www.237142.com | www.038677.com | 082053.com | 352863.com | www.57749.com | www.965541.com | www.7036.pw | www.742822.com | www.979163.com | www.903036.com | www.623753.com | www.0866.xyz | www.5635.cn | www.750776.com | www.306737.com | 716.cc | 9765.biz | 693595.com | www.320988.com | www.953632.com | www.775553.cc | 19pj.cc | 917307.com | 706599.com | www.820977.com | 997462.com | www.968497.com | www.406897.com | www.658536.com | www.944211.com | www.561600.com | 068765.com | 347820.com | 604747.com | www.78603.com | www.36520.cc | www.8wi.com | www.76zs.com | 352808.com | www.863134.com | www.993040.com | 026314.com | 0226.cc | 565236.com | www.893342.com | www.354534.com | www.437456.com | www.6851.org | 10182.com | 021044.com | www.595.date | www.bq20.com | www.165666.com | www.1527.vip | www.82857.cc | www.69ks.com | www.em16.com | www.01618.com | www.1264.cn | 彩票大家乐www.21202j.com | 974006.com | www.772479.com | www.794528.com | www.939880.com | 032184.com | www.337.red | www.81nm.com | www.xo28.com | www.6560.vip | www.70pg.com | 500彩票www.339968.com | 彩票驿站www.2350l.com | www.005629.com | www.130491.com | www.513014.com | www.653777.com | www.800433.com | 527522.com | www.953908.com | www.712676.com | www.699246.com | www.532589.com | www.237957.com | www.295289.com | www.609583.com | www.943458.com | 467448.com | www.595009.com | www.73tv.com | www.334077.com | www.413965.com | www.791574.com | bm35.cc | 802304.com | www.kl50.com | www.009437.com | www.123411.cc | www.028754.com | www.963666.cc | www.421990.com | www.272540.com | www.367926.com | 016006.com | www.827214.com | www.811626.com | www.59711.cc | www.006081.com | www.15750.com | www.2012.net | www.01ep.com | www.00fb.com | www.5995.top | www.505866.com | 364.com | www.949977.com | www.600906.com | 杏彩www.00840t.com | www.999796.com | www.61779.cc | www.4997.biz | www.1777.cm | www.02203.com | www.29jr.com | 323788.com | 953463.com | 663389.cc | www.74fa.com | www.214211.com | www.39413.com | www.258187.com | www.155214.com | www.111553.com | www.35999.com | www.147744.com | www.463332.com | www.818226.com | www.539307.com | www.961800.com | www.y42.biz | www.9575.org | www.48748.com | www.42901.com | www.37215.com | www.263677.com | www.259244.com | www.206405.com | www.23771.cc | www.883038.com | www.753711.com | www.333999.pw | www.817614.com | www.652247.com | www.835925.com | www.933995.com | www.925229.com | www.981719.com | www.hs2.com | www.8219.xyz | www.kj8.cc | www.cp5759.com | 983437.com | www.692175.com | www.303650.com | www.435573.com | www.712517.com | www.812500.com | www.620540.com | www.955642.com | www.621809.com | www.839277.com | www.678269.com | 954612.com | www.874896.com | www.124.tv | www.603867.com | www.78vx.com | www.eg83.com | 578253.com | www.879823.com | www.998974.com | www.860965.com | www.029015.com | www.027954.com | www.083162.com | www.175952.com | www.546365.com | www.247420.com | www.417898.com | www.914371.com | 943443.com | www.982938.com | www.48483.com | www.357.net | www.75zd.com | www.212964.com | www.381823.com | 彩票坊www.022cq.cc | www.148464.com | www.263307.com | www.525256.com | 018075.com | www.053833.com | www.666008.com | www.905339.com | 954890.com | www.4299.cc | www.04ey.com | www.mo88.cc | www.39785.com | www.2489.in | 842658.com | 4225.biz | 964644.com | 687313.com | www.28er.com | 302161.com | 华彩网www.www.hcw332.com | www.984040.cc | www.510392.com | uy28.com | www.58919.cc | www.731067.com | www.706007.com | www.381944.com | www.492688.com | www.412613.com | www.537954.com | www.792499.com | www.383504.com | www.981251.com | www.468664.com | www.969671.com | www.765637.com | www.972819.com | www.567162.com | www.964270.com | www.745.in | www.935131.com | www.741818.com | www.379656.com | www.621193.com | 7972.biz | www.5240.com | www.sw75.com | 072068.com | www.da39.com | www.686222.com | www.276665.com | www.32450.cc | www.380894.com | 505153.com | 050896.com | 158655.com | www.891533.com | www.825530.com | www.61769.cc | www.89970.cc | www.01238.cc | www.13ja.cc | www.553311.com | www.061494.com | 199481.com | www.883403.com | www.880353.com | www.921850.com | www.465444.com | www.111755.com | www.618525.com | www.640062.com | www.953493.com | www.719092.com | www.709570.com | www.302405.com | www.70bh.com | 590092.com | www.mr63.com | www.2335.biz | www.410817.com | 015634.com | 026460.com | www.913270.com | www.859816.com | 九五彩票www.95111w.com | 华彩网www.hcw332.com | 1310.pw | www.67vl.com | 770528.com | www.768063.com | www.cp1113.com | www.ak57.com | 947174.com | www.255067.com | www.35495.com | www.509390.com | www.800574.com | www.987718.com | 930262.com | www.77074.com | www.ve91.com | www.760775.com | www.82259.com | 03544.com | www.443651.com | www.u18.cx | www.582733.com | 367952.com | 007029.com | www.838359.com | 643377.com | www.939811.com | www.771361.com | www.871431.com | 353701.com | www.20xs.com | 500彩票www.50024o.com | www.263616.com | 554408.com | 772373.com | www.87828.com | www.305577.com | www.92677.cc | www.yv99.com | 福彩www.26878c.com | 528717.com | www.946695.com | www.481438.com | www.079669.com | www.36770.com | www.010803.com | www.52oj.com | 501909.com | 166492.com | 438741.com | www.974961.com | www.581127.com | www.570328.com | www.886418.com | www.01647.com | www.va65.com | 博友彩www.977657.com | www.972963.com | www.481476.com | 661462.com | www.46042.com | www.7086.cn | www.681811.com | www.217611.com | www.9136.cc | 500彩票www.348177.com | 870872.com | 932355.com | 325129.com | www.665700.com | www.353077.com | www.147363.com | www.510.mobi | www.86dc.com | www.ii42.com | www.zk11.com | www.jz44.cc | 796204.com | 500彩票www.50026u.com | 106980.com | www.854608.com | 959743.com | 661205.com | www.8336.loan | ki81.com | www.010972.com | www.480.cm | www.oc90.com | www.sw0.cc | 944474.com | www.807815.com | www.748311.com | www.c07.top | 323208.com | www.947114.com | www.920966.com | www.745856.com | www.300049.com | www.945218.com | www.329082.com | www.2879.com | www.696156.com | www.8rl.com | www.776845.com | www.2627.com | 069906.com | www.89621.com | www.971287.com | www.s91.cc | 640072.com | www.950194.com | 7253.biz | www.613721.com | www.794653.cc | www.907936.com | www.532403.com | www.119271.com | www.57953.com | www.rt00.com | 0550.pw | www.392115.com | www.636340.com | www.039030.com | www.39492.com | www.504276.com | www.55141.cc | www.m19.net | www.598tm.com | www.419325.com | www.713409.com | www.870271.com | www.811563.com | www.467369.com | www.456989.com | www.71336.cc | www.60xv.com | 705058.com | www.920178.com | www.463009.com | www.99538.com | www.h33.tv | www.5622.com | www.937095.com | 646762.com | 011059.com | www.735696.com | www.403307.com | www.528795.com | 599140.com | www.903252.com | www.568898.com | www.516688.com | www.093696.com | www.185162.com | www.234553.com | www.17697.com | www.qg77.cc | www.177929.com | www.284073.com | www.101354.com | www.61tg.com | www.632201.com | www.5678.gg | www.760986.com | 569671.com | www.909963.com |